Examination of substrate binding residues in the [alpha]-subunit of human [beta]-hexosaminidase
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Examination of substrate binding residues in the [alpha]-subunit of human [beta]-hexosaminidase

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Published by National Library of Canada in Ottawa .
Written in English


Book details:

Edition Notes

Thesis (M.Sc.) -- University of Toronto, 2002.

SeriesCanadian theses = -- Th`eses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative.
ID Numbers
Open LibraryOL21789834M
ISBN 100612687651
OCLC/WorldCa54414965

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The term ‘subsite’ represents an arrangement of amino acid residues that binds a single glycosyl residue of the polymeric substrate (Suganuma et al., ). Similar conclusions were drawn for a rice β-d-glucosidase, although the individual binding energies at each subsite were somewhat different (Opassiri et . The crystal structures of the two proteins represent the only examples in CBM fam and studies that evaluate the roles of amino acid residues in ligand binding in this family are lacking. In this study, we probed the roles of amino acids (selected based on CBM/ligand co . The formation of an enzyme–substrate complex is the first step in enzymatic catalysis The active sites of enzymes have some common features The binding energy between enzyme and substrate is important for catalysis The Michaelis–Menten Model Accounts for the Kinetic Properties of Many Enzymes Kinetics is the study of reaction rates. Asp is a Ser in the sturgeon, and the DS mutant of human thrombin has impaired Na ϩ binding and substrate recognition (10). Asp is the least conserved residue among the four.

Although the amino acid sequence of SmARG is only 42% identical with that of human arginase I, residues important for substrate binding and catalysis are strictly conserved. These peptide sequences determine the binding partners of each protein. One of the more prominent domains is the SH2 domain. SH2 domains play a vital role in cellular communication. Its length is approximately amino acids long and it is found within human proteins. Regarding its structure, it contains 2 alpha helices and 7 beta ro: IPR   The elements of this lesson which receive the greatest emphasis are: (1) the amino acid residues that comprise the binding domain of the active site cavity, (2) the vulnerability of a key SER residue at the edge of the active site to irreversible acetylation by aspirin, 6, 9 and (3) the structural differences between the allosteric binding Cited by:   Many carbohydrate-binding proteins contain aromatic amino acid residues in their binding sites. with the fact that this residue is the least abundant in human proteome (%, based site on the non-catalytic domain C of barley alpha-amylase participates in substrate binding and activity. FEBS J. ; – Cited by:

Platelet-derived growth factor receptors (PDGF-R) are cell surface tyrosine kinase receptors for members of the platelet-derived growth factor (PDGF) family. PDGF subunits -A and -B are important factors regulating cell proliferation, cellular differentiation, cell growth, development and many diseases including cancer. There are two forms of the PDGF-R, alpha and beta each encoded by a InterPro: IPR Structure of N-acetyl-[beta]-D-glucosaminidase adhesin of Streptococcus gordonii that mediates binding of this organism to human platelets via its interaction with sialyl-T antigen on the receptor GPIb{alpha}. the first crystal structures of {alpha}1,6-FucT in complex with its substrate GDP-Fuc and with GDP, which is a byproduct of the.   “The Biochemistry Questions Site” has been reviewed in the ASBMB website Posted on by biochemistryquestions This blog has been honoured with a review by Dr. Aditis Das, a Science writer and Research Scientist who provides reviews about related scientific blogs for the American Society for Biochemistry and Molecular Biology. Pomalidomide is distributed in semen of healthy subjects at a concentration of approximately 67% of plasma level at 4 hours post-dose (approximate Tmax) after 4 days of once-daily dosing at 2 mg. Human plasma protein binding ranges from 12% to 44% and is not concentration dependent. Pomalidomide is a substrate for P-glycoprotein (P-gp).